Hela cells can be grown in MEM media with carbon dioxide and with essential amino acids at 37 degrees;

Medium: 90% of MEM (with Earle's salts) with 10% FCS + 2 mM L-glutamine + non-essential amino acids. Can also use RPMI-1640 and 5-10% FBS.

Subculture: split confluent culture 1:4 or 1:6 every 3-5 days depending on confluency using trypsin/EDTA. Rate of doubling time is 24 to 48 hours. Seed at 1-2 x 106 cells/80 cm2. Incubate at 37 °C with 5% CO2

Storage: frozen with 70% medium, 20% FCS with 10% DMSO at about 1 x 106cells/ampoule

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Always Expect The Unexpected
Always Expect The Unexpected is a title of a new book which describes the start and early days of the Parachute Regiment and becoming world famous.

The humans know that they can't fly and therefore naturally scared of fallen, especially from great heights. Even sudden drops in altitude in modern aeroplane makes stomach churning.