Click here to promote your blog, your site, your products or services on this site for free, and earn money at the same time!

2

Bookmark and Share HeLa Cell Culture

HeLa Cell Culture
http://helacells.blogspot.com

Hela cells can be grown in MEM media with carbon dioxide and with essential amino acids at 37 degrees;

Medium: 90% of MEM (with Earle's salts) with 10% FCS + 2 mM L-glutamine + non-essential amino acids. Can also use RPMI-1640 and 5-10% FBS.

Subculture: split confluent culture 1:4 or 1:6 every 3-5 days depending on confluency using trypsin/EDTA. Rate of doubling time is 24 to 48 hours. Seed at 1-2 x 106 cells/80 cm2. Incubate at 37 °C with 5% CO2

Storage: frozen with 70% medium, 20% FCS with 10% DMSO at about 1 x 106cells/ampoule

Read more » HeLa Cell Culture
Rudra's picture
Published 2 weeks 6 days ago
Category: Science | Tags:
2

Bookmark and Share Mob Mentality Explained

Mob Mentality Explained
http://www.infobarrel.com

Suppose you are with two of your friends, Laura and Jim, after a football game and want to decide on where to eat afterward. Laura suggests you go to Burger King; however, she doesn't like their burgers and only suggests it because she knows Jim likes their fries.

Read more » Mob Mentality Explained
drbonemonkey's picture
Published 1 week 6 days ago
Category: Science | Tags:
2

Bookmark and Share Purchase Purple Comforters Online

Purchase Purple Comforters Online
http://hubpages.com

If you are looking to purchase a purple comforter, you will find the best deals online. You will find a better selection to choose from and the prices are usually less than those in department stores. Visit our website for a large selection of purple comforters with deeply discounted online prices.

Read more » Purchase Purple Comforters Online
HealthyWealthyWise's picture
Published 2 days 19 hours ago
Category: Science | Tags:
2

Bookmark and Share Glucocorticoid Receptor

Glucocorticoid Receptor
http://hubpages.com

Glucocortocoid (cortisol) is controlled by ACTH (adreno-cortocotropic hormone) produced by the anterior pituitary. ACTH secretion is in turn regulated by CRH (corticotrophin-releasing hormone) of the hypothalamus.

Read more » Glucocorticoid Receptor
Rudra's picture
Published 2 weeks 4 days ago
Category: Science | Tags:
2

Bookmark and Share Always Expect The Unexpected -- Parachute Regiment

Always Expect The Unexpected -- Parachute Regiment
http://hubpages.com

Always Expect The Unexpected
Always Expect The Unexpected is a title of a new book which describes the start and early days of the Parachute Regiment and becoming world famous.

The humans know that they can't fly and therefore naturally scared of fallen, especially from great heights. Even sudden drops in altitude in modern aeroplane makes stomach churning.

Read more » Always Expect The Unexpected -- Parachute Regiment
Bima.Purnawan's picture
Published 1 week 4 days ago
Category: Science | Tags:





 

Web Hosting Free Trial

Dreamhost
$50 OFF ANY PLAN WITH
PROMO CODE: SheToldMe


Or a limited offer, $100 off a 2-year plan!
Only 4 promo codes left:
215535142356
380140170026
443367978683
240642032963
Signup here.

Recent comments